4F9E

Cyclic di-GMP Sensing via the Innate Immune Signaling Protein STING

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-12-15
Detector	PSI PILATUS 6M
Wavelength(s)	0.9788
Spacegroup name	P 43 21 2
Unit cell lengths	61.400, 61.400, 118.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.001 - 2.750
R-factor	0.2138
Rwork	0.212
R-free	0.25750
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.202
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	SOLVE
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.001	35.001	2.820
High resolution limit [Å]	2.750	12.300	2.750
Rmerge		0.015	0.456
Total number of observations		558	3932
Number of reflections	6371
<I/σ(I)>	33	41.3	1.7
Completeness [%]	99.9	96	100
Redundancy	8.2	6	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1