4F99
Human CDC7 kinase in complex with DBF4 and nucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.480, 61.480, 237.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.520 - 2.330 |
| R-factor | 0.19396 |
| Rwork | 0.192 |
| R-free | 0.24148 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3PVG (Automatic molecular replacement with BALBES). |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.140 | 2.390 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.108 | 0.684 |
| Number of reflections | 20628 | |
| <I/σ(I)> | 15.4 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.1 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 25% PEG-1500, 15% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
