4F8X
Penicillium canescens endo-1,4-beta-xylanase XylE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | KURCHATOV SNC BEAMLINE K4.4 |
| Synchrotron site | KURCHATOV SNC |
| Beamline | K4.4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9887 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.230, 60.290, 55.620 |
| Unit cell angles | 90.00, 115.20, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.470 |
| R-factor | 0.14683 |
| Rwork | 0.146 |
| R-free | 0.16980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cui |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.807 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.330 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Number of reflections | 51675 | |
| <I/σ(I)> | 26.3 | 3.8 |
| Completeness [%] | 98.9 | 88.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | Protein solution (2mcl): 10mg/ml XylE, deionized water. Reservoir solution (2mcl): 0.1 M HEPES, 22% w/v PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
