4F8D
Crystal Structure of an R46A mutant of the Restriction-Modification Controller Protein C.Esp1396I (Monoclinic Form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979494 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 46.400, 51.000, 74.600 |
| Unit cell angles | 90.00, 96.60, 90.00 |
Refinement procedure
| Resolution | 24.700 - 1.500 |
| R-factor | 0.1783 |
| Rwork | 0.177 |
| R-free | 0.20020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.033 |
| RMSD bond angle | 2.633 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | |
| Low resolution limit [Å] | 24.700 | 30.000 |
| High resolution limit [Å] | 1.500 | 20.000 |
| Rmerge | 0.040 | 0.016 |
| Number of reflections | 27204 | 6 |
| <I/σ(I)> | 15.31 | 38.97 |
| Completeness [%] | 97.8 | 54.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 277 | 200mM Sodium Sulphate, 20% PEG 3350, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
