4F8A
Cyclic nucleotide binding-homology domain from mouse EAG1 potassium channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9765 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 60.346, 60.346, 85.415 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.261 - 2.200 |
| R-factor | 0.1907 |
| Rwork | 0.189 |
| R-free | 0.22010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.109 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.300 | 2.310 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.089 | 0.251 |
| Number of reflections | 9566 | |
| Completeness [%] | 99.4 | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 277 | 0.2 M tri-sodium citrate di-hydrate, 20% (w/v) PEG 3350, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
