4F88
X-ray Crystal Structure of PlyC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-03 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.853, 117.423, 222.192 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.720 - 3.300 |
| R-factor | 0.267 |
| Rwork | 0.266 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f87 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.700 | 3.480 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.100 | 0.658 |
| Number of reflections | 40690 | |
| Completeness [%] | 99.2 | 96.7 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 0.1 M Hepes, 12% PEG4000, 10% isopropanol, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
