4F7T
Crystal Structure of HLA-A*2402 Complexed with a Newly Identified Peptide from 2009 H1N1 PB1 (498-505)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-11-13 |
| Detector | RIGAKU RAXIS VII |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.838, 66.583, 70.362 |
| Unit cell angles | 97.70, 101.03, 111.84 |
Refinement procedure
| Resolution | 24.420 - 1.700 |
| R-factor | 0.197 |
| Rwork | 0.196 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i6l |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.912 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.045 | 0.289 |
| Number of reflections | 90267 | |
| <I/σ(I)> | 25.58 | 4.264 |
| Completeness [%] | 96.1 | 93.8 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 293 | 0.1 M Bis-Tris and 10% (w/v) polyethylene glycol 3,350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
