4F6R

Tubulin:Stathmin-like domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	95.060, 75.570, 155.680
Unit cell angles	90.00, 96.29, 90.00

Refinement procedure

Resolution	45.450 - 2.640
R-factor	0.1778
Rwork	0.177
R-free	0.20090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ryc 2xee
RMSD bond length	0.010
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.550	2.780
High resolution limit [Å]	2.640	2.640
Number of reflections	32036
<I/σ(I)>	10.5	2.1
Completeness [%]	98.7	97.5
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	PEG, MES BUFFER, PH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K