4F6P
Crystal structure of the yeast metacaspase Yca1 C276A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-12 |
Detector | RAYONIX MX325HE |
Wavelength(s) | 0.9793 |
Spacegroup name | H 3 |
Unit cell lengths | 114.400, 114.400, 61.679 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.009 - 1.619 |
R-factor | 0.179 |
Rwork | 0.179 |
R-free | 0.18530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f6o |
RMSD bond length | 0.007 |
RMSD bond angle | 1.061 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.680 |
High resolution limit [Å] | 1.619 | 1.620 |
Number of reflections | 37675 | |
Completeness [%] | 98.1 | 93.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.8M potassium phosphate monobasic, 0.8M sodium phosphate monobasic, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |