4F6N
Crystal structure of Kaiso zinc finger DNA binding protein in complex with methylated CpG site DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-25 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 44.049, 183.402, 230.138 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.000 - 2.800 |
| R-factor | 0.23009 |
| Rwork | 0.228 |
| R-free | 0.26148 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Kaiso from complex with Kaiso binding site protein |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.579 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.087 | 0.581 |
| Number of reflections | 11780 | |
| <I/σ(I)> | 23 | 3.3 |
| Completeness [%] | 99.3 | 92.4 |
| Redundancy | 7.36 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 1M NaCl, 0.1M sodium cacodylate, 10% glycerol, 30% Peg 600, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
