4F65
Crystal structure of Human Fibroblast Growth Factor Receptor 1 Kinase domain in complex with compound 8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-02-08 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 210.713, 50.365, 65.641 |
| Unit cell angles | 90.00, 106.67, 90.00 |
Refinement procedure
| Resolution | 39.950 - 2.260 |
| R-factor | 0.2043 |
| Rwork | 0.203 |
| R-free | 0.23550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.929 | 2.380 |
| High resolution limit [Å] | 2.259 | 2.259 |
| Rmerge | 0.041 | 0.270 |
| Number of reflections | 30388 | |
| <I/σ(I)> | 21.3 | 4.7 |
| Completeness [%] | 96.2 | 80.17 |
| Redundancy | 2.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 16-20% PEG8000, 100 mM PCTP, 100-300 mM ammonium sulfate, 25% ethylene glycol, pH 6.25-7.25, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
