4F64
Crystal structure of Human Fibroblast Growth Factor Receptor 1 Kinase domain in complex with compound 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-12-23 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 208.810, 57.277, 65.433 |
| Unit cell angles | 90.00, 107.55, 90.00 |
Refinement procedure
| Resolution | 61.900 - 2.050 |
| R-factor | 0.21701 |
| Rwork | 0.214 |
| R-free | 0.26912 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fgk |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.403 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.931 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.149 | 0.572 |
| Number of reflections | 44839 | |
| <I/σ(I)> | 6.6 | 2.2 |
| Completeness [%] | 96.8 | 96.06 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 16-20% PEG8000, 100 mM PCTP, 100-300 mM ammonium sulfate, 25% ethylene glycol, pH 6.25-7.25, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
