4F5V
Crystal Structure of Leporine Serum Albumin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9400 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.202, 79.644, 104.223 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.270 |
| R-factor | 0.19621 |
| Rwork | 0.193 |
| R-free | 0.25768 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f5s 1ao6 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.929 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.109 | 0.499 |
| Number of reflections | 27095 | |
| <I/σ(I)> | 15.3 | 1.72 |
| Completeness [%] | 93.1 | 63 |
| Redundancy | 6.4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG 3350, 0.2 M ammonium acetate, 0.1 M Tris pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
