4F5S
Crystal Structure of Bovine Serum Albumin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 217.800, 44.989, 143.057 |
| Unit cell angles | 90.00, 114.13, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.470 |
| R-factor | 0.19884 |
| Rwork | 0.197 |
| R-free | 0.25877 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ao6 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.006 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.570 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.091 | 0.549 |
| Number of reflections | 43883 | |
| <I/σ(I)> | 11.4 | 1.75 |
| Completeness [%] | 95.2 | 84.9 |
| Redundancy | 4.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% mmePEG 5000, 0.25M ammonium chloride, 0.1M MES 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
