4F59
Triple mutant Src SH2 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 114 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-04-26 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 |
| Unit cell lengths | 67.070, 67.070, 46.910 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.280 - 1.710 |
| R-factor | 0.2064 |
| Rwork | 0.204 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.536 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.281 | 27.281 | 1.790 |
| High resolution limit [Å] | 1.706 | 5.360 | 1.700 |
| Rmerge | 0.040 | 0.264 | |
| Total number of observations | 1880 | 9054 | |
| Number of reflections | 13044 | ||
| <I/σ(I)> | 26.2 | 15.3 | 2.8 |
| Completeness [%] | 97.0 | 80.4 | 85.9 |
| Redundancy | 5.8 | 5.1 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 277 | 0.1 M Tris-HCl, 18% PEG6000, 0.2 M lithium chloride, pH 7.6, vapor diffusion, sitting drop, temperature 277K |
