4F58
Fab structure of a neutralizing antibody L3 from an early subtype A HIV-1 infected patient
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-11-27 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.03319 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.403, 126.413, 120.082 |
| Unit cell angles | 90.00, 90.08, 90.00 |
Refinement procedure
| Resolution | 46.500 - 2.488 |
| R-factor | 0.2105 |
| Rwork | 0.208 |
| R-free | 0.25640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f57 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.165 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.500 | 2.540 |
| High resolution limit [Å] | 2.488 | 2.488 |
| Number of reflections | 76041 | |
| Completeness [%] | 99.8 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | 28% PEG 4K, 0.17 M Li2SO4, 0.085 M Tris pH 8.5, and 15% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
