4F4Y
Y-family DNA polymerase chimera Dbh-Dpo4-Dbh
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.076, 103.390, 112.988 |
| Unit cell angles | 90.00, 101.76, 90.00 |
Refinement procedure
| Resolution | 29.250 - 2.338 |
| R-factor | 0.2234 |
| Rwork | 0.222 |
| R-free | 0.25860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.110 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (in PHENIX) |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.390 |
| High resolution limit [Å] | 2.338 | 2.350 |
| Rmerge | 0.084 | 0.245 |
| Number of reflections | 47630 | |
| <I/σ(I)> | 16.5 | 3.9 |
| Completeness [%] | 95.3 | 80.7 |
| Redundancy | 2.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 298 | 10% PEG-3350, 100 mM Ca(OAc)2, 100 mM HEPES, 2.5% Glycerol, 250mM sucrose, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
