4F4X
Y-family DNA polymerase chimera Dbh-Dpo4-Dpo4 #2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 101.122, 102.306, 52.879 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.735 - 2.049 |
R-factor | 0.2196 |
Rwork | 0.218 |
R-free | 0.25030 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.323 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (in PHENIX) |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.080 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.070 | 0.333 |
<I/σ(I)> | 24.4 | 5.1 |
Completeness [%] | 97.3 | 80.9 |
Redundancy | 4.9 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 10% PEG-3350, 100 mM Ca(OAc)2, 100 mM MES/Tris, 2.5% Glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |