4F4L
Open Channel Conformation of a Voltage Gated Sodium Channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8726 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.070, 74.080, 107.930 |
| Unit cell angles | 90.00, 118.26, 90.00 |
Refinement procedure
| Resolution | 43.620 - 3.490 |
| R-factor | 0.2713 |
| Rwork | 0.271 |
| R-free | 0.28520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rvy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.220 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 95.100 | 95.000 | 3.900 |
| High resolution limit [Å] | 3.490 | 7.800 | 3.490 |
| Rmerge | 0.048 | 0.025 | 0.717 |
| Number of reflections | 8747 | ||
| <I/σ(I)> | 22.98 | 77.75 | 3.29 |
| Completeness [%] | 83.1 | 96.4 | 47.8 |
| Redundancy | 11.1 | 10.3 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 277 | 30% PEG 400, 0.09 M tri-sodium citrate, pH 5.6, VAPOR DIFFUSION, temperature 277K |
