4F4L

Open Channel Conformation of a Voltage Gated Sodium Channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	C 1 2 1
Unit cell lengths	117.070, 74.080, 107.930
Unit cell angles	90.00, 118.26, 90.00

Refinement procedure

Resolution	43.620 - 3.490
R-factor	0.2713
Rwork	0.271
R-free	0.28520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rvy
RMSD bond length	0.010
RMSD bond angle	1.220
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	95.100	95.000	3.900
High resolution limit [Å]	3.490	7.800	3.490
Rmerge	0.048	0.025	0.717
Number of reflections	8747
<I/σ(I)>	22.98	77.75	3.29
Completeness [%]	83.1	96.4	47.8
Redundancy	11.1	10.3	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6	277	30% PEG 400, 0.09 M tri-sodium citrate, pH 5.6, VAPOR DIFFUSION, temperature 277K