4F48
The X-ray structural of FimXEAL-c-di-GMP-PilZ complexes from Xanthomonas campestris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 158.222, 158.222, 64.808 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.560 - 3.000 |
| R-factor | 0.249 |
| Rwork | 0.249 |
| R-free | 0.25000 |
| Structure solution method | SAD |
| RMSD bond length | 0.032 |
| RMSD bond angle | 4.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 27412 | |
| Completeness [%] | 99.4 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1M HEPES pH 7.5, 20% PEG 3000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
