4F48
The X-ray structural of FimXEAL-c-di-GMP-PilZ complexes from Xanthomonas campestris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 158.222, 158.222, 64.808 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.560 - 3.000 |
R-factor | 0.249 |
Rwork | 0.249 |
R-free | 0.25000 |
Structure solution method | SAD |
RMSD bond length | 0.032 |
RMSD bond angle | 4.900 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 27412 | |
Completeness [%] | 99.4 | 99.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1M HEPES pH 7.5, 20% PEG 3000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |