4F3Z
Crystal structure of a swine H1N2 influenza virus hemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-01-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.440, 94.920, 220.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.275 - 3.200 |
| R-factor | 0.237 |
| Rwork | 0.234 |
| R-free | 0.29330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lzg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.757 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.165 | |
| Number of reflections | 32867 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 98.2 | 85.7 |
| Redundancy | 7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 296 | 25% PEG1000, 0.1 M Tris, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
