4F3D
Structure of RPE65: P65 crystal form grown in Fos-Choline-10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 65 |
| Unit cell lengths | 175.258, 175.258, 86.449 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.800 - 2.500 |
| R-factor | 0.19395 |
| Rwork | 0.193 |
| R-free | 0.21896 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3fsn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.091 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.7.0025) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.113 | 0.882 |
| Number of reflections | 52159 | |
| <I/σ(I)> | 10.1 | 1.53 |
| Completeness [%] | 99.1 | 98.8 |
| Redundancy | 3.7 | 3.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 281 | 35% 2-ethoxyethanol, 22 mM Fos-choline-10, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
