4F3A
Structure of RPE65: P6522 crystal form, iridium derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0952 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 178.639, 178.639, 86.469 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.440 - 2.600 |
| R-factor | 0.18734 |
| Rwork | 0.185 |
| R-free | 0.22868 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.354 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.7.0025) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.105 | 0.898 |
| Number of reflections | 25186 | |
| <I/σ(I)> | 19 | 2.15 |
| Completeness [%] | 98.7 | 91.8 |
| Redundancy | 10.6 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 281 | 11% PEG3350, pH 8, crystal soaked in 1 mM iridium hexachloride, VAPOR DIFFUSION, SITTING DROP, temperature 281K |
