4F35
Crystal Structure of a bacterial dicarboxylate/sodium symporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-15 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.330, 100.870, 164.568 |
| Unit cell angles | 90.00, 101.74, 90.00 |
Refinement procedure
| Resolution | 44.377 - 3.196 |
| R-factor | 0.2304 |
| Rwork | 0.228 |
| R-free | 0.29130 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.557 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.520 | 3.270 |
| High resolution limit [Å] | 3.196 | 3.196 |
| Number of reflections | 54103 | |
| Completeness [%] | 97.9 | 83.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 29% PEG 1000, 0.05M Lithium citrate, 0.05M MOPS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
