4F2B
Modulation of S.Aureus Phosphatidylinositol-Specific Phospholipase C Membrane Binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-12-19 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.362, 133.382, 50.190 |
| Unit cell angles | 90.00, 89.95, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.160 |
| R-factor | 0.21673 |
| Rwork | 0.215 |
| R-free | 0.25047 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v16 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 2.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.220 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.092 | |
| Completeness [%] | 96.8 | 99.7 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 20% PEG 4000, 0.150 M ammonium acetate, 0.100 M sodium acetate, 0.001 M magnesium nitrate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
