4F26
Crystal structure of the second RRM domain of human PABPC1 a pH 9.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9772 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.955, 69.487, 41.787 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.800 - 2.000 |
| R-factor | 0.21866 |
| Rwork | 0.216 |
| R-free | 0.28606 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cvj |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.789 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.077 | 0.279 |
| Number of reflections | 5162 | |
| <I/σ(I)> | 16.9 | 5.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 294 | 25% PEG 1500 and 0.1 M MMT buffer , pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
