4F25
Crystal structure of the second RRM domain of human PABPC1 at pH 6.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-04 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9772 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 50.653, 50.653, 63.447 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.050 - 1.900 |
R-factor | 0.21915 |
Rwork | 0.217 |
R-free | 0.26919 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cvj |
RMSD bond length | 0.010 |
RMSD bond angle | 1.218 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 7194 | |
<I/σ(I)> | 16.9 | 5.3 |
Completeness [%] | 97.7 | 82.7 |
Redundancy | 9.5 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 25% PEG 1500 and 0.1 M MIB buffer, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |