4F1U
Subatomic resolution structure of a high affinity periplasmic phosphate-binding protein (PfluDING) bound with phosphate at pH 4.5
Replaces: 3G62Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Detector technology | CCD |
| Collection date | 2006-12-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.953 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.960, 124.370, 41.010 |
| Unit cell angles | 90.00, 116.55, 90.00 |
Refinement procedure
| Resolution | 36.690 - 0.980 |
| R-factor | 0.08814 |
| Rwork | 0.088 |
| R-free | 0.09591 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3G62 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.216 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.690 |
| High resolution limit [Å] | 0.980 |
| Number of reflections | 185558 |
| Completeness [%] | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 4% PEG 8000, 100 mM acetate buffer pH 4.5, 200 mM Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
