4F1K
Crystal structure of the MG2+ free VWA domain of plasmodium falciparum trap protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 89.434, 89.434, 104.232 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.870 |
| R-factor | 0.19013 |
| Rwork | 0.188 |
| R-free | 0.23663 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.601 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.100 | 0.645 |
| <I/σ(I)> | 11.49 | 2.24 |
| Completeness [%] | 99.6 | 98 |
| Redundancy | 4.28 | 4.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 25% PEG 3350, 0.1M HEPES, 0.2M lithium sulfate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
