Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 2001-02-09 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.386, 77.607, 145.411 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.200 - 2.200 |
| R-factor | 0.1963 |
| Rwork | 0.194 |
| R-free | 0.23940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 3EVH |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.369 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.200 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 38230 | |
| <I/σ(I)> | 13.2 | 2.8 |
| Completeness [%] | 99.4 | 97.5 |
| Redundancy | 5.6 | 2.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 24%(W/V) PEG4000, 0.3M AMMONIUM ACETATE, 0.1M SODIUM CITRATE, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
