4F0Y
Crystal structure of aminoglycoside antibiotic 6'-N-acetyltransferase AAC(6')-IG from Acinetobacter haemolyticus, apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-07 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.541780 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.963, 44.018, 46.576 |
| Unit cell angles | 83.19, 87.44, 72.65 |
Refinement procedure
| Resolution | 24.073 - 2.560 |
| R-factor | 0.1839 |
| Rwork | 0.181 |
| R-free | 0.24010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s3z |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.655 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.610 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Number of reflections | 8914 | |
| <I/σ(I)> | 8.205 | 2.095 |
| Completeness [%] | 96.9 | 94.6 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.2 M magnesium chloride, 0.1 M Bis-Tris, pH 5.5, 25% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
