4F0S

Crystal structure of an adenosine deaminase homolog from Chromobacterium violaceum (target NYSGRC-019589) with bound inosine.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97880
Spacegroup name	P 41 21 2
Unit cell lengths	101.048, 101.048, 88.004
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.343 - 1.851
R-factor	0.158
Rwork	0.157
R-free	0.18490
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.082
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.880
High resolution limit [Å]	1.850	1.850
Number of reflections	39235
<I/σ(I)>	21.9	4.3
Completeness [%]		98.6
Redundancy	11.4	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	298	2% dioxane, 0.1M bicine PH 9.0, 10% PEG 20000, VAPOR DIFFUSION, SITTING DROP, temperature 298K