4F0Q
MspJI Restriction Endonuclease - P21 Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-23 |
| Detector | MAR 225 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.764, 144.284, 87.842 |
| Unit cell angles | 90.00, 116.27, 90.00 |
Refinement procedure
| Resolution | 34.569 - 2.046 |
| R-factor | 0.2119 |
| Rwork | 0.210 |
| R-free | 0.24580 |
| Structure solution method | AB INITIO PHASING |
| Starting model (for MR) | HgSAD phased MspJI |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.590 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.7.3_928) |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.570 | 2.120 |
| High resolution limit [Å] | 2.046 | 2.050 |
| Rmerge | 0.138 | 0.651 |
| Number of reflections | 122248 | |
| <I/σ(I)> | 14.6 | 3.8 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 8.5 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 289 | 4% PEG 3350, 100 mM MgCl2, 100 mM imidazole, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
