4F0B
Crystal structure of the glutathione transferase URE2P1 from Phanerochaete chrysosporium.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.980557 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.907, 53.947, 165.225 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.169 - 1.450 |
| R-factor | 0.142 |
| Rwork | 0.141 |
| R-free | 0.16490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gxo |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.456 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.650 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.026 | 0.262 |
| Number of reflections | 77259 | |
| <I/σ(I)> | 24.9 | 7.3 |
| Completeness [%] | 94.7 | 81.3 |
| Redundancy | 7.4 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.6 | 277 | 20% (v/v) 2-propanol, 20% (w/v) PEG-4000, 0.1M sodium citrate tribasic dihydrate, pH 5.6, MICROBATCH, temperature 277K |
