4F01
Crystal structure of an artificial dimeric DnaK complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.110, 61.380, 69.190 |
| Unit cell angles | 90.00, 103.39, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.400 |
| R-factor | 0.16329 |
| Rwork | 0.162 |
| R-free | 0.21260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.982 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.140 | 1.470 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmerge | 0.049 | 0.423 |
| Number of reflections | 97037 | |
| Completeness [%] | 98.7 | 98 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 292 | 2.9 M ammonium sulfate 0.1 M MES pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
