4F00
Crystal structure of the substrate binding domain of E.coli DnaK in complex with an apidaecin fragment from the bumblebee (residues 3 to 11)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.918 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 94.156, 116.201, 37.169 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.950 |
R-factor | 0.19493 |
Rwork | 0.192 |
R-free | 0.24220 |
Structure solution method | PDB ID 1DKZ |
RMSD bond length | 0.018 |
RMSD bond angle | 1.940 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | REFMAC (with PDBID 1DKZ) |
Refinement software | REFMAC (with PDBID 1DKZ) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.056 | 0.401 |
Number of reflections | 15317 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 292 | 2.4 M sodium malonate 0.1 M citric acid pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |