4EZ2
Crystal Structure of d(CCGGGACCGG)4 as a four-way junction at 1.6 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.010, 24.350, 38.180 |
| Unit cell angles | 90.00, 112.53, 90.00 |
Refinement procedure
| Resolution | 19.305 - 1.600 |
| R-factor | 0.2179 |
| Rwork | 0.216 |
| R-free | 0.24870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 467d |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.259 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.520 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.054 | 0.268 |
| Number of reflections | 7323 | |
| <I/σ(I)> | 11.7 | 3.6 |
| Completeness [%] | 99.3 | 99 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 50mM sodium cacodylate trihydrate buffer (pH 7.0), 10mM MnCl2, 1mM spermine, 50% methyl pentane diol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
