4EYY

Crystal Structure of the IcmR-IcmQ complex from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-01
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 2
Unit cell lengths	68.948, 113.411, 49.465
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.750 - 2.400
R-factor	0.222
Rwork	0.217
R-free	0.26200
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.721
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.754	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.208	0.341
Number of reflections	15596
<I/σ(I)>	20.8	5.4
Completeness [%]	98.0	99.1
Redundancy	7.4	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1			294	Protein concentration 8 mg/ml. Selenomethionine-incorporated protein for SAD phasing. 1:1 ratio of protein to 24% PEG 1500, 0.1 M sodium citrate pH 5.9, 1 mM DTT and 1% ethylene glycol. Cryoprotected in mother liquor + 30% ethylene glycol and frozen in liquid nitrogen, VAPOR DIFFUSION, HANGING DROP, temperature 294K
2