4EYU

The free structure of the mouse C-terminal domain of KDM6B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-11
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	82.977, 102.284, 145.302
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.450 - 2.300
R-factor	0.232
Rwork	0.230
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.145
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.297
Rmerge	0.072
Number of reflections	55090
Completeness [%]	99.3
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.2	298	11% methanol, 6% MPD, 5% PEG 4K, 5% GLYCEROL, HEPES pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K