4EYT

Crystal structure of the C-terminal domain of Tetrahymena telomerase protein p65

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	C 1 2 1
Unit cell lengths	156.190, 91.900, 82.830
Unit cell angles	90.00, 107.36, 90.00

Refinement procedure

Resolution	60.549 - 2.500
R-factor	0.2269
Rwork	0.225
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4erd
RMSD bond length	0.009
RMSD bond angle	1.192
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	79.057	60.549	2.640
High resolution limit [Å]	2.500	7.910	2.500
Rmerge		0.028	0.577
Total number of observations		7682	18272
Number of reflections	36719
<I/σ(I)>	11.6	20.2	1.3
Completeness [%]	94.5	94.7	93.5
Redundancy	6.2	6.3	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	278	0.1 M phosphate/citrate, 0.2 M lithium sulfate, 20% w/v PEG1000, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 278K