4EYS
Crystal structure of MccF-like protein from Streptococcus pneumoniae in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 97.998, 97.998, 79.805 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.077 - 1.580 |
| R-factor | 0.17036 |
| Rwork | 0.169 |
| R-free | 0.19756 |
| Structure solution method | SAD/MR |
| Starting model (for MR) | 4e94 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.996 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.079 | |
| Number of reflections | 60681 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.2 M Ca(oAc)2, 0.1 M imidazol, 10% Peg 8000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
