4EYC
Crystal structure of the cathelin-like domain of human cathelicidin LL-37 (hCLD)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 91.146, 57.987, 39.546 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.1938 |
| Rwork | 0.191 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pfp |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.067 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.930 |
| Rmerge | 0.372 | |
| Number of reflections | 16740 | |
| <I/σ(I)> | 6.1 | |
| Completeness [%] | 99.4 | 98.7 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1 m SODIUM HEPES, 0.2 m SODIUM CITRATE, 20% ISOPROPANOL, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
