4EYA
Crystal Structure of a Plectonemic RNA Supercoil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0622 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.424, 126.264, 144.131 |
| Unit cell angles | 90.00, 89.98, 90.00 |
Refinement procedure
| Resolution | 39.974 - 3.200 |
| R-factor | 0.2295 |
| Rwork | 0.228 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3r2d |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.549 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 6.670 | 3.100 |
| Rmerge | 0.095 | 0.034 | 0.457 |
| Number of reflections | 37610 | ||
| <I/σ(I)> | 7.4 | ||
| Completeness [%] | 90.8 | 100 | 53.1 |
| Redundancy | 6.7 | 7.5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2M ammonium sulfate, 26% PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
