4EYA

Crystal Structure of a Plectonemic RNA Supercoil

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-24
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0622
Spacegroup name	C 1 2 1
Unit cell lengths	126.424, 126.264, 144.131
Unit cell angles	90.00, 89.98, 90.00

Refinement procedure

Resolution	39.974 - 3.200
R-factor	0.2295
Rwork	0.228
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3r2d
RMSD bond length	0.002
RMSD bond angle	0.549
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.210
High resolution limit [Å]	3.100	6.670	3.100
Rmerge	0.095	0.034	0.457
Number of reflections	37610
<I/σ(I)>	7.4
Completeness [%]	90.8	100	53.1
Redundancy	6.7	7.5	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.2M ammonium sulfate, 26% PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K