4EXK
A chimera protein containing MBP fused to the C-terminal domain of the uncharacterized protein STM14_2015 from Salmonella enterica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.079, 64.079, 180.912 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.280 |
| R-factor | 0.14324 |
| Rwork | 0.141 |
| R-free | 0.17772 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hpi |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.203 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.300 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.057 | |
| Number of reflections | 103107 | |
| <I/σ(I)> | 28.5 | |
| Completeness [%] | 92.1 | 76 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.2 M Ammonium Acetate, 25 % peg 3320, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
