4EX4
The Structure of GlcB from Mycobacterium leprae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-03 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.610, 59.740, 118.460 |
| Unit cell angles | 90.00, 100.97, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.18341 |
| Rwork | 0.181 |
| R-free | 0.23423 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n8i |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.539 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 | |
| High resolution limit [Å] | 2.100 | 9.390 | 2.100 |
| Rmerge | 0.100 | 0.022 | 0.430 |
| Number of reflections | 85731 | ||
| <I/σ(I)> | 12.24 | 51.58 | 3.17 |
| Completeness [%] | 99.7 | 95.2 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | MyleA18344aA1 PW34800 19.28mg/ml, 100mM HEPES pH7.5, 20%w/v PEG4000, 10% Isopropanol. Cryoprotection 20% ethylene glycol. , VAPOR DIFFUSION, SITTING DROP, temperature 298K |
