4EWR

X-ray structure of WDR5-SETd1a Win motif peptide binary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Detector technology	CCD
Collection date	2008-08-14
Detector	ADSC QUANTUM 210
Wavelength(s)	0.917
Spacegroup name	C 2 2 21
Unit cell lengths	78.494, 98.659, 80.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.665 - 1.503
R-factor	0.1741
Rwork	0.173
R-free	0.19780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.162
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.550
High resolution limit [Å]	1.500	3.230	1.500
Rmerge	0.100	0.044	0.634
Number of reflections	49713
<I/σ(I)>	9.3
Completeness [%]	99.7	99.6	98.9
Redundancy	13.6	14.2	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	7.6	294	PEG3350, Ammonium Sulfate, HEPES, pH 7.6, hanging drop, temperature 294K