4EWJ

structure of the enloase from Streptococcus suis serotype 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-11-22
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.54178
Spacegroup name	I 4
Unit cell lengths	168.016, 168.016, 77.104
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.028 - 2.403
R-factor	0.1405
Rwork	0.139
R-free	0.16230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w6t
RMSD bond length	0.002
RMSD bond angle	0.520
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.062	0.312
Number of reflections	41166
<I/σ(I)>	46.9	7.2
Completeness [%]	98.3	96.8
Redundancy	7.2	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.1M MES (pH 6.5), 10% (w/v) PEG 20000, VAPOR DIFFUSION, HANGING DROP, temperature 277K