4EW6

Crystal structure of D-galactose-1-dehydrogenase protein from Rhizobium etli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-15
Detector	PSI PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	52.109, 52.109, 265.344
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.900 - 2.300
R-factor	0.24612
Rwork	0.244
R-free	0.29331
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.614
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.068	0.444
Number of reflections	17360
<I/σ(I)>	11.7
Completeness [%]	100.0	100
Redundancy	18.2	18.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.2M Sodium Acetate, 0.1M Tris HCl, 30% PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K