4EVX
Crystal structure of putative phage endolysin from S. enterica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792914 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 54.400, 54.400, 261.090 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.070 - 1.700 |
| R-factor | 0.1959 |
| Rwork | 0.195 |
| R-free | 0.21490 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.074 | 0.667 |
| Number of reflections | 26172 | |
| <I/σ(I)> | 23.2 | 2.43 |
| Completeness [%] | 98.7 | 100 |
| Redundancy | 6.6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | 0.14 M CaCl2, 0.07 M Na acetate/HCl, 14% 2-propanol, 30% glycerol, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
