4EVW
Crystal Structure of the nucleoside-diphosphate-sugar pyrophosphorylase from Vibrio cholerae RC9. Northeast Structural Genomics Consortium (NESG) Target VcR193.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-18 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.303, 117.003, 128.561 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.247 - 1.900 |
R-factor | 0.185 |
Rwork | 0.184 |
R-free | 0.20300 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.334 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SnB |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.130 | 0.535 |
Number of reflections | 111375 | |
<I/σ(I)> | 25.44 | 3.16 |
Completeness [%] | 99.9 | 100 |
Redundancy | 10.4 | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch crystallization under oil | 7.5 | 277 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: 20% PEG 8000, 0.1M magnesium nitrate, 0.1M HEPES, pH 7.5, Microbatch crystallization under oil , temperature 277K |